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Sterile Lab Skills: Agar and Liquid Culture

intermediate About 2 hours

Topics covered

  • Agar plates
  • Liquid culture
  • Strain isolation
  • Contamination ID
  • Culture storage

Curriculum

  1. 1OverviewWhy work in a lab· Preview10 min
  2. 2AgarPouring and reading agar25 min
  3. 3AgarClean transfers and strain isolation25 min
  4. 4Liquid cultureMaking and maintaining liquid culture25 min
  5. 5MaintenanceStoring and refreshing cultures15 min

About this course

Who this is for

This course suits growers who can already fruit a block and want to control their own genetics. It focuses on the lab skills that keep strains clean and strong.

What you will learn

You will learn to pour and work on agar, make and maintain liquid culture, isolate a single healthy strain, and recognise contamination early. These skills let you multiply cultures cheaply and keep them pure over time.

What you need

Agar plates, a scalpel and flame, liquid culture jars, and a still air box or flow hood. Careful, patient technique matters more than expensive equipment.

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